2009. Identification and fine-mapping genomic regions associated with plant regeneration response in maize (Zea mays L.)

2009. Identification and fine-mapping genomic regions associated with plant regeneration response in maize (Zea mays L.)

DOWNLOAD: PDF
SÐT: 0981800855 (A. LONG)
PRICE: 100.000 VND
EMAIL: FOODCROPS@GMAIL.COM

ABSTRACT

Maize (Zea mays L.) is an important model organism for scientific advances in crop improvement and answering basic genetic and biological questions. Genetic engineering is an invaluable research tool for exploiting extensive genetic resources available to maize researchers for scientific advancement. Utilization of genetic engineering leads to advances in maize production and basic knowledge of plant genetic and biological systems. Application of genetic engineering in the study of maize is hindered, however, by the requirement for tissue culture as part of the genetic engineering process. Only a few genotypes of maize demonstrate high culture and regeneration response suitable for genetic engineering. The genotype-dependent tissue culture response of maize greatly impedes progress in functional genomics studies, and adds years to genetics research and crop improvement efforts. The goal of this research is to identify and characterize maize genes significantly associated to tissue culture response. Identification and characterization of genes associated with tissue culture response will likely lead to the development of new maize culture and transformation systems that are genotype-independent.

Two inbred maize lines, A188 (high Type-II culturability and regenerability; poor agronomic performance), and B73 (poor culturability/regenerability; excellent agronomic performance), formed the basis of the mapping populations used in this research. Results show chromosome 3 (3.07) has a QTL associated with plant regeneration response in a 3.58 cM PHM13673.53-umc2050 marker interval. SSR marker umc2050 accounts for 20.0% of the total variation, and the marker interval contains ~1.9 Mb of genomic sequence. Further analysis revealed an additional significant plant regeneration QTL on chromosome 4 (4.10) and a putative plant regeneration QTL on chromosome 2 (2.08). Future evaluation of the chromosome 3 (3.07) 1.9 Mb sequence interval, chromosomes 2 (2.08), and 4 (4.10) will likely yield genetic factors responsible for enhancing plant regeneration response in recalcitrant lines. Additionally, lines derived from the A188xB73 mapping population, which are ~95 - 99% B73, could be suitable for use in tissue culture and transformation experiments. Lastly, identification and characterization of genetic factors regulating plant regeneration response will increase the likelihood of developing a line-independent maize tissue culture and transformation system  .